Sequencing symbiotic genes in an EMS-mutagenized Aeschynomene evenia population

A population of Aeschynomene evenia plants (CIAT22838) was mutagenized with ethylmethanesulfonate (EMS) and screened for mutations in candidate nodulation genes using Targeted Sequence Capture followed by Next-Generation Sequencing and mutation discovery.

研究人员以乙基甲磺酸酯（ethylmethanesulfonate, EMS）对敏感合萌（Aeschynomene evenia）种质CIAT22838的群体开展诱变处理，随后依次通过靶向序列捕获（Targeted Sequence Capture）、下一代测序（Next-Generation Sequencing）与突变发现流程，针对候选结瘤基因的突变进行筛选。