Physiological response and proteomics analysis of Castanopsis fissa under drought stress

Castanopsis fissa is an evergreen broad-leaved species of the cone genus Castanopsis in the family Fagaceae, which is widely distributed and is an excellent native species in Guangdong Province of China. This species has a well-developed root system, excellent soil-fixing power, and better soil and water conservation ability and has the characteristics of barren tolerance, strong sprouting power, abundant and easily decomposed dead leaves, etc. Therefore, C. fissa is not only a pioneer species for postdestruction sprouting forests but also a highly potential ecological public welfare forest tree species. Moreover, due to its beautiful shape, wide canopy and various colors, it has become an ideal tree for landscaping and ornamental purposes. However, there is a basic gap in knowledge in the reports on the drought resistance or drought tolerance genes of C. fissa. Based on the above details, in this study, 2-year-old C. fissa seedlings were used as the study material to investigate the physiological response under drought stress by a potted drought experiment, and we also compared and analyzed the differentially expressed proteins (DEPs) under different periods of drought stress by TMT quantitative labeling protein to prepare a preliminary study on the physiological response and proteomic mechanism of C. fissa adaptation to drought stress.

黧蒴锥（Castanopsis fissa）是壳斗科（Fagaceae）锥属（Castanopsis）的常绿阔叶树种，分布范围广泛，是中国广东省的优良乡土树种。该物种根系发达、固土能力优异，水土保持功能良好，同时具备耐贫瘠、萌芽力强、枯落物丰富且易分解等特性。因此，黧蒴锥不仅是植被破坏后萌芽林的先锋树种，同时也是极具开发潜力的生态公益林树种。此外，其株形优美、冠幅开阔且叶色多样，亦成为园林绿化与景观观赏的理想树种。然而，目前关于黧蒴锥抗旱或耐旱相关基因的研究报道仍存在显著认知空白。基于上述背景，本研究以2年生黧蒴锥实生苗为试验材料，通过盆栽控水干旱试验探究其在干旱胁迫下的生理响应；同时采用TMT定量标记蛋白质组学技术，对不同干旱胁迫时段下的差异表达蛋白（DEPs）进行比较分析，以期为揭示黧蒴锥适应干旱胁迫的生理响应机制与蛋白质组学调控机制奠定前期研究基础。