RNASeq analysis for EBV-immortalized B-cells from individuals with an AIOLOS E82K mutation and healthy controls. RNASeq analysis for EBV-immortalized B-cells from individuals with an AIOLOS E82K mutation and healthy controls

RNA-seq was performed using RNA extracted from EBV-immortalized B-cells for the following study groups: healthy controls (HC, n=3) and patients with AIOLOSE 82K (n=4 ). Illumina libraries were generated using the SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian Components (Takara San Jose, CA). Libraries were sequenced on a Illumina NextSeq 2000 instrument using 2 x 75 bp paired-end reads, and alignment and mapping analyses were performed by the Takara Cogent NGS Analysis Pipeline v1.5.0 (also known as CogentAP). Differential gene expression analysis was performed by DESeq2 v1.32.0 [Bioconductor version: Release (3.15)]. Overall design: Comparative gene expression profiling analysis of RNA-seq data for EBV-immortalized B-cells from individuals with an AIOLOS E82K mutation and healthy controls

本研究采用从爱泼斯坦-巴尔病毒（Epstein-Barr virus, EBV）永生化B细胞中提取的RNA开展RNA测序（RNA-seq），研究分为两组：健康对照组（healthy controls, HC，n=3）以及携带AIOLOS E82K突变的患者组（n=4）。使用SMARTer链特异性总RNA测序试剂盒v3——低起始量哺乳动物样本组分（Takara，美国加利福尼亚州圣何塞）构建Illumina测序文库。测序文库在Illumina NextSeq 2000测序仪上进行2×75 bp双端测序，序列比对与映射分析采用Takara Cogent NGS分析流程v1.5.0（亦称CogentAP）完成。差异基因表达分析采用DESeq2 v1.32.0软件完成[Bioconductor版本：正式版（3.15）]。实验整体设计：对携带AIOLOS E82K突变个体及健康对照的EBV永生化B细胞的RNA-seq数据开展比较基因表达谱分析。