CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity [aCD3 stimulation]

Integrative system approaches uncover a three-tier system for the regulatory modules of nutrient signaling-dependent mTORC1 activation in primary T cells We used Microarray to compare gene expression of sgNTC with sgSmarcb1 transduced iTreg

整合系统方法揭示了原代T细胞中依赖营养信号通路的哺乳动物雷帕霉素靶蛋白复合物1（mTORC1）激活调控模块的三级调控体系。本研究采用基因芯片（Microarray）技术，对比分析了转导非靶向单引导RNA对照（sgNTC）与靶向Smarcb1基因的单引导RNA（sgSmarcb1）的诱导性调节性T细胞（iTreg）的基因表达水平差异。