Expression data from control-knockdown (CTR-KD) and WWC1-KD human multiple myeloma (MM) cell lines

To determine what signalling pathways are affected by WWC1(WW Domain-Containing Protein 1) in MM cells, we established CTR-KD and WWC1-KD ARD MM cell lines. Total RNA of each sample was extracted using TRIzol Reagent (Invitrogen). Next generation sequencing libraries were constructed using a standard RNA library preparation kit for Illumina. RNA-seq was performed by Majorbio (China) on an Illumina NovaSeq X Plus platform.

为探究WWC1（含WW结构域蛋白1，WW Domain-Containing Protein 1）对MM细胞中哪些信号通路的调控作用，我们构建了CTR-KD与WWC1-KD的ARD MM细胞系。采用TRIzol试剂（Invitrogen）提取所有样本的总RNA。使用适配Illumina平台的标准RNA文库制备试剂盒构建下一代测序文库。RNA测序（RNA-seq）由中国的美吉生物（Majorbio）在Illumina NovaSeq X Plus测序平台完成。