Respiration rates, photosynthetic efficiency, and mortality of brooded coral larval experiments, March 2011 and 2012, Taiwan (Cumbo, 2013) (MCR LTER project, Climate_Coral_Larvae project)

<p>To evaluate the effects of temperature and pCO2 on coral larvae, brooded larvae of Pocillopora damicornis from Nanwan Bay, Taiwan (21°56.179' N, 120°44.85' E), were exposed to ambient (419-470 µatm) and high (604-742 µatm) pCO2 at ~25 and ~29 °C in two experiments conducted in March 2010 and March 2012. Larvae were sampled from four consecutive lunar days (LD) synchronized with spawning following the new moon, incubated in treatments for 24 h, and measured for respiration, maximum photochemical efficiency of PSII (F v/F m), and mortality.</p>

<p>The most striking outcome was a strong effect of time (i.e., LD) on larvae performance: respiration was affected by an LD × temperature interaction in 2010 and 2012, as well as an LD × pCO2 × temperature interaction in 2012; F v/F m was affected by LD in 2010 (but not 2012); and mortality was affected by an LD × pCO2 interaction in 2010, and an LD × temperature interaction in 2012. There were no main effects of pCO2 in 2010, but in 2012, high pCO2 depressed metabolic rate and reduced mortality. Therefore, differences in larval performance depended on day of release and resulted in varying susceptibility to future predicted environmental conditions. These results underscore the importance of considering larval brood variation across days when designing experiments. Subtle differences in experimental outcomes between years suggest that transgenerational plasticity in combination with unique histories of exposure to physical conditions can modulate the response of brooded coral larvae to climate change and ocean acidification.</p>

<p>These data include dark respiration rates, maximum photochemical efficiency of PSII (F v/F m), and mortality&nbsp;from the experimental tanks, March 2011 and 2012.</p>

<p><strong>Related datasets:</strong></p>

<p><a href=\"http://www.bco-dmo.org/dataset/528806\">brooded coral larvae 2 - carbonate chemistry</a><br />
<a href=\"http://www.bco-dmo.org/dataset/528880\">brooded coral larvae 2 - larval release March 2003-2008</a></p>

<p>These data are published in Vivian R Cumbo, Peter J Edmunds, Christopher B Wall, Tung-Yung Fan. (2013) Brooded coral larvae differ in their response to high temperature and elevated pCO2 depending on the day of release. Marine Biology. See Figures 1 and 2.</p>

<p><a href=\"http://dmoserv3.whoi.edu/data_docs/Climate_Coral_Larvae/Cumbo_etal_2013_MarBio_BCODMO.xlsx\" target=\"_blank\">Download complete data for this publication (Excel file)</a><br />
Data also available from PANGAEA: <a href=\"http://doi.pangaea.de/10.1594/PANGAEA.831612\" target=\"_blank\">DOI 10.1007/s00227-013-2280-y</a></p>

为评估温度与二氧化碳分压（pCO₂）对珊瑚幼虫的影响，研究人员于2010年与2012年3月开展了两组实验，将采自台湾南湾（21°56.179' N, 120°44.85' E）的鹿角杯形珊瑚（Pocillopora damicornis）育幼幼虫，分别置于~25℃与~29℃环境下，暴露于环境水平（419-470 µatm）与高浓度（604-742 µatm）的pCO₂中。幼虫采自与新月后产卵同步的连续四个朔望月日（LD），经24小时处理孵育后，测定其呼吸速率、光系统II（PSII）的最大光化学效率（Fv/Fm）及死亡率。

本研究最显著的结果为时间（即朔望月日LD）对幼虫性能的强烈调控作用：2010年与2012年的呼吸速率均受LD×温度交互作用影响，2012年还同时受LD×pCO₂×温度三重交互作用调控；2010年光系统II最大光化学效率Fv/Fm受LD影响（2012年未观察到此效应）；死亡率在2010年受LD×pCO₂交互作用影响，2012年则受LD×温度交互作用调控。2010年未观测到pCO₂的主效应，但2012年高浓度pCO₂可抑制幼虫代谢速率并降低死亡率。由此可见，幼虫性能的差异取决于其释放日期，且对未来预测的环境条件的敏感性存在异质性。本研究结果强调，在设计相关实验时，需考虑不同日期产出的育幼型珊瑚幼虫种群间的差异。不同年份实验结果的细微差异表明，跨代可塑性结合独特的物理环境暴露历史，可调控育幼型珊瑚幼虫对气候变化与海洋酸化的响应。

本数据集包含2011年3月与2012年实验养殖箱中的暗呼吸速率、光系统II最大光化学效率（Fv/Fm）及死亡率数据。

**相关数据集：**
<a href="http://www.bco-dmo.org/dataset/528806">育幼型珊瑚幼虫数据集2——碳酸盐化学特征</a><br />
<a href="http://www.bco-dmo.org/dataset/528880">育幼型珊瑚幼虫数据集2——2003-2008年3月幼虫释放记录</a>

本数据已发表于Vivian R Cumbo、Peter J Edmunds、Christopher B Wall、童永樊（Tung-Yung Fan）团队2013年发表于《海洋生物学》的论文《育幼型珊瑚幼虫对高温与高pCO₂的响应因释放日期而异》，详见论文中的图1与图2。

可通过<a href="http://dmoserv3.whoi.edu/data_docs/Climate_Coral_Larvae/Cumbo_etal_2013_MarBio_BCODMO.xlsx" target="_blank">下载本研究完整数据（Excel文件）</a>，数据亦可从PANGAEA数据库获取：<a href="http://doi.pangaea.de/10.1594/PANGAEA.831612" target="_blank">DOI 10.1007/s00227-013-2280-y</a>