Labcode 2- HIV neutralization testing with Luciferase Reporter Gene Assay in TZM-BL Cells

This protocol describes a pseudovirus assay that measures neutralization of HIV, SIV and SHIV as a function of reductions in Tat-regulated Luc reporter gene expression after a single round of infection in TZM-bl cells.

本实验方案详述了一种假病毒试验（pseudovirus assay），该体系可在TZM-bl细胞中完成一轮感染后，通过检测Tat调控的Luc报告基因表达量的下调幅度，定量评估针对人类免疫缺陷病毒（HIV）、猴免疫缺陷病毒（SIV）以及猴-人免疫缺陷病毒（SHIV）的中和活性。