Nerve growth factor on hippocampal mitochondria in VD rats: TMT Proteomic analysis of Data

We used Sprague-Dawley rats to establish VD model, and used the proteomic method based on relative quantification (iTRAQ) to identify the differentially expressed proteins in hippocampus mitochondria. A total of 33 differentially expressed proteins were identified between the VD rats and the VD rats treated with nerve growth factor groups. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis were done after that.

本研究以斯普拉格-道利（Sprague-Dawley）大鼠为实验对象，构建血管性痴呆（VD）模型；采用基于相对定量的同位素标记相对和绝对定量（iTRAQ）蛋白质组学方法，鉴定海马线粒体中的差异表达蛋白。在血管性痴呆模型大鼠组与经神经生长因子干预的血管性痴呆模型大鼠组间，共鉴定得到33种差异表达蛋白。随后开展了基因本体（Gene Ontology，GO）分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）信号通路富集分析。