Angiogenesis, the phosphoinositide 3-kinase and the Wnt signaling pathways may mediate primary resistance to chemo-immunotherapy in advanced gastric cancer patients

Diagnostic endoscopic biopsies were retrospectively obtained from advanced GC patients previously categorized as responders (R) or non-responders (NR) to chemo-immunotherapy. Thirty-four tumor biopsies (R n=16, NR n=18) were analyzed by 3 prime- massive analysis of cDNA ends (3MACE). We found more than 30 differentially expressed genes between R and NRs. Subsequent pathway enrichment analyses demonstrated that angiogenesis and the Wnt-b-catenin signaling pathway were enriched in NRs

本研究回顾性采集了既往被划分为化学免疫治疗应答者（R组）与非应答者（NR组）的晚期胃癌（Gastric Cancer, GC）患者的诊断性内镜活检标本。共纳入34份肿瘤活检标本（R组n=16，NR组n=18），采用cDNA 3'端大规模分析（3MACE）进行检测。本研究发现R组与NR组间存在超过30个差异表达基因。后续通路富集分析表明，血管生成及Wnt-β-连环蛋白信号通路（Wnt-β-catenin）在NR组中显著富集。