Sugar dataset final.xlsx

Single source white clover (Fabaceae: <i>Trifolium repens</i>) pollen pellets collected by <i>Apis mellifera</i> purchased from Ames Farm (Watertown, Minnesota, USA; www.amesfarm.com) and single source rose (Rosaceae: <i>Rosa </i>spp<i>.</i>) pollen collected by <i>Apis mellifera</i> purchased from the China Health Club (Chongqing, China; www.teaherbalshop.com) were used to create artificial diets to raise <i>C. calcarata</i> eggs. Pollen identity and purity were based on vendor claims. Sugar samples were thawed in 50°C water bath for 3 min and then centrifuged at &gt;2,500 rpms for 5 min. Sugar content was measured in degrees Brix by placing 20 mL of the supernatant on a handheld refractometer. Based on the weight of the original pollen ball, we determined the milligrams of sugar in each sample.

本研究所用花粉材料包含两类：其一为购自美国明尼苏达州沃特敦市Ames Farm（www.amesfarm.com）、由西方蜜蜂（Apis mellifera）采集的单源白三叶（豆科Fabaceae：白车轴草Trifolium repens）花粉团；其二为购自中国重庆China Health Club（www.teaherbalshop.com）、由西方蜜蜂（Apis mellifera）采集的单源玫瑰（蔷薇科Rosaceae：蔷薇属Rosa spp.）花粉。上述花粉被用于制备人工饲料，以饲养距螯蜂（C. calcarata）的卵。花粉的种类与纯度均以供应商标注为准。糖样品经50℃水浴解冻3分钟后，以≥2500转/分钟的转速离心5分钟；取20mL上清液置于手持折光仪中，以白利糖度（degrees Brix）值表征糖含量。依据原始花粉团的重量，可计算得到每份样品中的糖含量（单位：毫克）。