p53 independent TAF6delta target genes.. Homo sapiens

To elucidate the impact of TAF6d on cell death and gene expression, here we have employed modified antisense oligonucleotides to enforce expression of endogenous TAF6d. The induction of endogenous TAF6d triggered apoptosis in tumor cell lines, including cells devoid of p53. Microarray experiments revealed that TAF6d activates gene expression independently of cellular p53 status. Our data define TAF6d as a pivotal node in a signaling pathway that acts immediately downstream of p53 to control gene expression programmes and apoptosis. Keywords: Treatment with splice site switching antisense oligonucleotides, induction of apoptosis Overall design: Biological triplicates, 4 conditions: isogenic p53+/+ and -/- cells; control oligonucleotide, TAF6 AS2 oligonucleotide

为阐明TAF6d对细胞死亡与基因表达的影响，本研究采用修饰型反义寡核苷酸（modified antisense oligonucleotides）诱导内源性TAF6d的表达。内源性TAF6d的诱导表达可在多种肿瘤细胞系中触发细胞凋亡，包括p53缺陷型细胞。基因芯片（microarray）实验结果显示，TAF6d对基因表达的激活作用不受细胞内p53状态的影响。本研究数据表明，TAF6d是一条信号通路中的关键节点，该通路直接作用于p53下游，以调控基因表达程序与细胞凋亡。
关键词：剪接位点切换型反义寡核苷酸处理、细胞凋亡诱导
实验设计：设置3次生物学重复，共4组实验条件：同遗传背景的p53野生型（p53+/+）与p53缺陷型（p53-/-）细胞，分别经对照寡核苷酸与TAF6 AS2寡核苷酸处理