Staphylococcus aureus drives expansion of low-density neutrophils in diabetic mice

Diabetes is associated with an increase in neutrophil NET production. Low density neutrophils (LDNs) have a greater propensity for spontaneous production of NETs, and are increased in the diabetic host during infection with S. aureus. We compared gene expression between high density (HDN) and LDNs isolated from the blood of infected db/db mice. Mice were infected with 5e7 CFU S. aureus (SF8300) and blood was collected 48h post infection. Blood from 10 mice were pooled and LDNs and HDNs purified. Matched pairs of LDN and HDN were compared.

糖尿病与中性粒细胞胞外陷阱（Neutrophil Extracellular Traps, NETs）的生成增加密切相关。低密度中性粒细胞（Low density neutrophils, LDNs）自发产生NETs的倾向性更强，且在感染金黄色葡萄球菌（S. aureus）的糖尿病宿主中数量增多。本研究对比了从感染db/db小鼠血液中分离的高密度中性粒细胞（High density neutrophils, HDNs）与LDNs的基因表达。实验中，小鼠经5×10⁷菌落形成单位（Colony-Forming Unit, CFU）的S. aureus菌株SF8300感染，并于感染后48小时采集血液。将10只小鼠的血液混合后纯化得到LDNs与HDNs，随后对匹配的LDN-HDN样本对开展基因表达对比分析。