Expression data from mouse spleens after experimental stroke. Mus musculus

Infection is a major complication and cause of mortality and morbidity after acute stroke however the mechanisms are poorly understood. After experimental stroke the microarchitecture and cellular composition of the spleen are extensively disrupted resulting in deficits to immune function. We used microarray to determine differentially expressed genes in the spleens of mice after experimental stroke to determine contributers to immunosuppression after stroke. Overall design: We extracted RNA from spleens from 3 mice 5 d after experimental stroke when we have shown peak disruption to cellular composition and also from spleens from 2 sham-operated control mice for comparison.

感染是急性脑卒中后的主要并发症，亦是致死与致残的重要诱因，但其潜在发病机制目前仍未得到充分阐明。在实验性脑卒中模型中，脾脏的微结构与细胞组成会受到广泛破坏，进而导致免疫功能受损。本研究采用基因芯片（microarray）技术，对实验性脑卒中模型小鼠的脾脏组织开展差异表达基因分析，以期明确脑卒中后免疫抑制的潜在促成因素。实验设计概述：我们分别采集了3只实验性脑卒中造模后5天（此时脾脏细胞组成破坏已达峰值）的小鼠脾脏组织，以及2只假手术对照小鼠的脾脏组织，提取总RNA以用于后续对比研究。