Multifunctional material enables ultrafast and large-scale enrichment of glycopeptides and reveals distinct spatial patterns in oxidative stress

Our study offers a convenient and robust site-specific chemproteomics tool for glycoproteome profiling, enabling simultaneous identification of O-GlcNAc sites and N-glycosites by a two-step enzymatic release strategy, and the application of this material revealed distinct spatial O-GlcNAcylation patterns upon oxidative stress.

本研究构建了一款便捷且稳定的位点特异性化学蛋白质组学（chemproteomics）工具，用于糖蛋白质组（glycoproteome）分析。该工具通过两步酶促释放策略，可同时鉴定O-连接N-乙酰葡糖胺（O-GlcNAc）修饰位点与N-糖基化位点。利用该工具开展的应用研究，揭示了氧化应激下特征鲜明的空间分布O-糖基化（O-GlcNAcylation）模式。