Loss of integrin αvβ8 on murine hepatocytes accelerates liver regeneration. Loss of integrin αvβ8 on murine hepatocytes accelerates liver regeneration

We assess the difference in overall gene expression levels post 2/3 hepatectomy in mouse livers as a result of loss of integrin αvβ8, through microArray experiments. We hypothesized that depletion of hepatocyte integrin αvβ8 would increase hepatocyte proliferation and accelerate liver regeneration following injury. Through use of microarray experiments we identify genes that are up/down regulated only in the mice with integrin αvβ8 depletion. Overall design: Four replicates of β8flox/flox / Alb-Cre- baseline adult livers (uninjured) were compared to four replicates of β8flox/flox / Alb-Cre- remnant liver following 2/3 hepatectomy, and four replicates of β8flox/flox / Alb-Cre+ baseline adult liver (uninjured) compared with four relicates of β8flox/flox / Alb-Cre+ remnant liver following 2/3 hepatectomy. All samples are 24 hours post hepatectomy.

本研究通过微阵列（microArray）实验，探究小鼠肝脏在2/3肝切除术后，整合素αvβ8缺失所引发的整体基因表达水平差异。我们提出研究假说：肝细胞整合素αvβ8的条件性敲除可促进肝细胞增殖，加速损伤后肝脏再生。本研究通过微阵列实验，筛选出仅在整合素αvβ8缺失小鼠中出现上调或下调的差异表达基因。实验整体设计如下：将4份β8flox/flox / Alb-Cre- 基因型的成年未损伤肝脏样本（基线对照组），与4份同基因型小鼠经2/3肝切除术后24小时采集的残留肝脏样本进行对比；同时将4份β8flox/flox / Alb-Cre+ 基因型的成年未损伤肝脏样本（基线对照组），与4份同基因型小鼠经2/3肝切除术后24小时采集的残留肝脏样本进行对比。所有样本均采集于肝切除术后24小时。