Dynamic Intracellular Metabolic Data of four human stem cells lines after an extracellular glutamine step increase

Four different human stem cell lines were exposed to an extracellular glutamine step increase. Intracellular metabolic concentration was measured before the step increase and after at several tine-point until 2 hours.
Two cell lines of hiPSC were used: IMR90-4 (RRID: CVCL_C437) from WiCell and WTC-11 (RRID:424 CVCL_Y803) from The J. David Gladstone Institutes.
Two cell lines of hiPSC-NSC were used: derived from hiPSC 1 IMR90-4 using a dual SMAD inhibition protocol and derived from hiPSC line RIi001-A (RRID: CVCL_C888).

将4种不同的人类干细胞系暴露于胞外谷氨酰胺浓度阶跃升高的环境中，分别于阶跃升高前及升高后的多个时间节点（直至2小时）检测其胞内代谢物浓度。
本次实验使用2株诱导多能干细胞（hiPSC）系：分别为来自WiCell研究所的IMR90-4（RRID: CVCL_C437），以及来自J·戴维·格拉德斯通研究所的WTC-11（RRID: CVCL_Y803）。
本次实验还使用2株诱导多能干细胞来源神经干细胞（hiPSC-NSC）系：一株通过双SMAD抑制方案从hiPSC IMR90-4诱导得到，另一株从hiPSC细胞系RIi001-A（RRID: CVCL_C888）中诱导得到。