shld1 effect on 3T3 cell

NIH3T3 cells were cultured in medium containing 1 uM, 100 nM, 10 nM and 0 nM (serve as control) shld1 for 24 hours. Three independent cultures were obtained for each drug concentration. Total RNA from experimental samples were extracted using RNeasy Kit (Qiagen). Mouse RNA pooled from 11 cell lines (Stratagene, La Jolla, CA) was used as reference. Cy5-dUTP and Cy3-dUTP, were used to label the experimental cDNA and the reference samples, respectively, and hybridized to the Mouse Exonic Evidence Based Oligonucleotide (MEEBO) arrays (http://www.microarray.org./sfgf/meebo). The hybridizations were performed by Stanford Functional Genome Facility using its standard protocol (Oligo Array Hybridization protocol, http://www.microarray.org./sfgf/docView.do?type=2). A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Keywords: compound_treatment_design Computed

将NIH3T3细胞接种于分别添加1 μM、100 nM、10 nM及0 nM（作为空白对照）shld1的培养基中培养24小时。每个浓度组设置三次独立生物学重复。采用RNeasy试剂盒（Qiagen）提取实验样本的总RNA；以从11株细胞系中混合制备的小鼠RNA（Stratagene，美国加利福尼亚州拉霍亚市）作为参考样本。分别使用Cy5-dUTP与Cy3-dUTP标记实验样本cDNA与参考样本cDNA，随后将标记产物与基于外显子证据的小鼠寡核苷酸（Mouse Exonic Evidence Based Oligonucleotide, MEEBO）芯片（http://www.microarray.org./sfgf/meebo）进行杂交。本次杂交实验由斯坦福功能基因组设施（Stanford Functional Genome Facility）按照其标准实验方案（寡核苷酸阵列杂交方案，http://www.microarray.org./sfgf/docView.do?type=2）完成。化合物处理设计类型指针对化合物或化学物质（包括激素类生物化合物）的给药响应开展检测的实验设计类型。关键词：化合物处理设计（compound_treatment_design） 已计算。