Shot gun sequencing of genomes of Iberian freshwater fish species

We produced a reference database of whole genome sequences of freshwater fish species from Portuguese rivers. We also included some species from other European countries. This database was made to serve as reference for eDNA metagenomic studies focusing on Portuguese fish taxa. To this end, genomes were sequenced with an average depth of 20 x using paired end Illumina sequencing (2 x 150 bp) and assembled with the program Abyss.

本研究构建了葡萄牙河流淡水鱼类物种的全基因组序列参考数据库，同时纳入了部分产自欧洲其他国家的鱼类物种。该数据库旨在作为针对葡萄牙鱼类类群的环境DNA（eDNA）宏基因组研究的参考依据。为此，研究采用双端Illumina测序（2×150 bp）策略对基因组进行测序，平均测序深度达20×，并使用Abyss软件完成基因组组装。