H3K27Me3 (Abcam2).BG3.CP190_RNAi

modENCODE_submission_3680 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The goal of these experiments are to validate and confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. To do this, we are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, and then using antibodies to perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody H3K27Me3 (Abcam2) (target is H3K27Me3); dsRNA (RNAi_reagent) CG6384_RNAi&oldid=39664

modENCODE_submission_3680 本提交源自Gary Karpen主持的modENCODE项目。如需查阅modENCODE项目完整清单，请访问http://www.genome.gov/26524648。项目目标：本系列实验旨在验证并确认黑腹果蝇（Drosophila melanogaster）基因组内125种染色体蛋白的结合位点。具体实验方案为：利用RNA干扰（RNA interference, RNAi）技术在果蝇BG3与S2组织培养细胞中敲降单个非组蛋白染色体蛋白，随后通过抗体介导的染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合基因组平铺芯片开展检测。对比目标蛋白因子在敲降前后的结合图谱，可显著提升预测结果的置信度。有关数据使用的条款与条件，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip（染色质免疫沉淀-芯片）。生物来源：细胞系：ML-DmBG3-c2；组织：中枢神经系统来源细胞系；发育阶段：三龄幼虫期；基因型：y v f mal；性别：未知；重复次数：4。实验因子：细胞系ML-DmBG3-c2；抗体：H3K27Me3（Abcam2）（靶标为H3K27Me3）；双链RNA（RNAi试剂）：CG6384_RNAi&oldid=39664