Evaluation of aquatic invasive species using eDNA at multiple sites in Oregon

A critical element in the management of public lands and native aquatic species is the identification and eradication of aquatic invasive species. Left unchecked, aquatic invasive species are known to have devastating ecological and economic effects. Early detection of invasive species is the key to population control before species become ubiquitous in colonized environments. To meet the need to monitor large water bodies for multiple invasive aquatic plant and animal species we developed a multi-species microfluidic metabarcoding array targeting 15 invasive plants and algae, 14 invasive animal species alongside 1 pathogen and 8 universal animal targets. We used this array to assess invasive species in navigable river reaches, lakes and reservoirs in sites throughout Oregon, USA, bi-weekly from June to October 2018 with an additional follow up survey in 2023.

公共土地与本土水生生物管理的核心环节之一，是水生入侵物种的识别与根除。已知若不加管控，水生入侵物种会造成极具破坏性的生态与经济后果。在入侵物种在定植生境中广泛扩散前，对其进行早期检测是实现种群防控的关键。为满足对大型水体中多种入侵水生动植物进行监测的需求，我们开发了一款多物种微流体元条形码阵列（microfluidic metabarcoding array），可靶向检测15种入侵植物与藻类、14种入侵动物物种，同时涵盖1种病原体与8种通用动物检测靶点。我们利用该阵列对美国俄勒冈州全境的通航河段、湖泊与水库开展入侵物种监测：2018年6月至10月期间每两周采样一次，并于2023年补充开展了一次追踪调查。