Gene expression profiles of human iPSC-derived microglia in monoculture and in co-culture with isogenic iPSC-derived neural cells

The goal of the study was to developed a new method for the rapid isolation of pure and intact phagosomes from human pluripotent stem cell-derived microglia under various, highly defined experimental conditions. Here, human pluripotent stem cell-derived microglia were either cultured in monocultures, or together with isogenic, human pluripotent stem cell-rerived neural cells

本研究旨在开发一种全新方法，可在多种高度明确的实验条件下，从人类多能干细胞诱导的小胶质细胞（human pluripotent stem cell-derived microglia）中快速分离得到纯净且完整的吞噬体（phagosomes）。本研究中，人类多能干细胞诱导的小胶质细胞既可进行单培养，也可与同基因的人类多能干细胞诱导的神经细胞共培养。