Substrate Stiffness Modulates the Growth, Phenotype, and Chemoresistance of Ovarian Cancer Cells. Substrate Stiffness Modulates the Growth, Phenotype, and Chemoresistance of Ovarian Cancer Cells

Purpose: Identify the effect of substrate stiffness on gene expression Methods:Evaluating for differentially expressed mRNAs in the SKOV-3 cells grown on the different substrates via High-throughput sequence Results: We found that the general direction of changes in gene expression of cells grown on the different substrates and the most significant signalling pathways and the expression of gene orthologs broadly involved in platinum drug resistance, apoptosis, cell cycle. Conclusions: Our study represents the first detailed analysis of the effects of substrate stiffness on gene expression of ovarian cancer cells. Overall design: SKOV-3 cells were seeded in the petri dishes, coated with hydrogel substrates of different stiffness (0.5kPa, 4kPa, 25kPa).

研究目的：明确基质刚度（substrate stiffness）对基因表达的影响。
研究方法：通过高通量测序技术，对培养于不同基质上的SKOV-3细胞内的差异表达mRNA进行检测分析。
研究结果：本研究发现，在不同刚度基质上培养的细胞，其基因表达的整体变化方向、最为显著的信号通路，以及广泛参与铂类药物耐药、细胞凋亡与细胞周期过程的基因同源物（gene orthologs）的表达均存在显著差异。
研究结论：本研究首次详细分析了基质刚度对卵巢癌细胞基因表达的影响。
整体实验设计：将SKOV-3细胞接种于包被有不同刚度（0.5kPa、4kPa、25kPa）水凝胶基质的培养皿中。