Gene expression comparisons of megakaryocyte-erythrocyte progenitors isolated from wild type and c-MybTG/c-MybTG mice. Mus musculus

Transgene insertion in proximity to the c-Myb gene disrupts c-Myb expression in megakaryocyte-erythrocyte progenitors (MEPs), resulting in skewed commitment to megakaryocyte lineage. To understand the genes regulated by c-Myb at the megakaryocyte-erythrocyte bifurcation, MEPs of c-MybTG/c-MybTG mice were examined. Detailed analysis on c-MybTG/c-MybTG is descried in the paper by Mukai et al, Mol Cell Biol 2006. Overall design: The IL-7R-Lin-Sca-1–c-Kit+ fraction was subdivided into FcR loCD34+, FcRloCD34-, and FcRhiCD34+ populations. Among them, FcRloCD34- fraction was use as MEPs. MEPs from 16 wild type mice and those from 32 c-MybTG/c-MybTG mice were pooled and used for RNA purification.

插入于c-Myb基因邻近区域的转基因会干扰巨核细胞-红细胞祖细胞（megakaryocyte-erythrocyte progenitors, MEPs）中c-Myb的表达，进而导致细胞向巨核细胞系的分化偏倚。为探究在巨核细胞-红细胞分化分支点受c-Myb调控的基因，本研究对c-MybTG/c-MybTG小鼠的MEPs进行了检测。关于c-MybTG/c-MybTG小鼠的详细分析，详见Mukai等人2006年发表于《分子与细胞生物学》的论文。实验整体设计：将IL-7R-Lin-Sca-1–c-Kit+细胞群进一步分为FcR loCD34+、FcRloCD34-及FcRhiCD34+三个亚群，其中FcRloCD34-亚群被用作MEPs。收集16只野生型小鼠及32只c-MybTG/c-MybTG小鼠的MEPs并混合，用于RNA纯化实验。