Baltic TRANSCOAST G3 project, 3rd cohort

Coastal wetlands can be seen as natural laboratories for the future impacts of sea-level rise and the intricacies of the effect of sulfate on emissions of the greenhouse gases methane and carbon dioxide. A 3-month anoxic incubation experiment was conducted with soil extracted from the Drammendorf peatland along the NE German Baltic coast, which was rewetted with brackish water 2 months prior to examine how the microbial community has adapted to the new conditions at the site. Soil slurries from three depths were incubated at a moderate temperature of 15 °C at two different salinities (a high and low value within current in-situ salinities) and sampled at 8 timepoints. Microbial community composition was evaluated via 16S rRNA amplicon sequencing and shotgun metagenomic sequencing (Illumina platform).

滨海湿地可被视为研究海平面上升未来影响，以及硫酸盐（sulfate）对温室气体（greenhouse gases）甲烷（methane）与二氧化碳（carbon dioxide）排放调控机制的天然实验室。本研究针对德国东北部波罗的海沿岸德拉门多夫泥炭地（Drammendorf peatland）采集的土壤开展了为期3个月的厌氧培养实验：该泥炭地在实验前2个月已通过微咸水（brackish water）完成复湿处理，旨在探究微生物群落（microbial community）如何适应该位点的新环境条件。实验选取三个土层深度的土壤悬液（soil slurries），在15℃的适中温度下，以两种分别处于当前原位盐度区间高低限值的盐度进行培养，并在8个时间点完成取样。研究通过16S rRNA扩增子测序（16S rRNA amplicon sequencing）与鸟枪法宏基因组测序（shotgun metagenomic sequencing，Illumina平台）对微生物群落组成进行解析。