西太平洋M4浅海山的五个站位的真核微生物原始数据集

该数据集来自西太平洋 M4 浅海海山区域五个站点的水体样品，提取了总 DNA 和 RNA后，使用真核微生物通用引物 TAReuk454FWD1 和 TAReukREV3 对 18S rRNA 基因的 V4 区进行扩增。每个样本均进行了三次重复的 PCR 扩增，以降低实验误差。扩增产物经质量检测合格后，使用 Illumina HiSeq 平台进行测序，获得原始序列数据。随后，使用 VSEARCH 进行引物去除和质量控制，并通过 USEARCH 进行去噪处理，最终得到扩增子序列变异（zOTUs）。所有 zOTUs 利用 pr2_5.0.0 数据库进行真核微生物分类注释。

This dataset is derived from water samples collected at five stations in the M4 shallow seamount region of the Western Pacific Ocean. After extracting total DNA and RNA, the V4 region of the 18S rRNA gene was amplified using the universal eukaryotic microbial primers TAReuk454FWD1 and TAReukREV3. Triplicate PCR amplifications were performed for each sample to reduce experimental errors. Following quality inspection of the amplified products, sequencing was conducted on the Illumina HiSeq platform to obtain raw sequence data. Subsequently, primer trimming and quality control were performed using VSEARCH, followed by denoising via USEARCH, ultimately yielding amplicon sequence variants (zOTUs). All zOTUs were taxonomically annotated against the pr2_5.0.0 database for eukaryotic microorganisms.