Gene expression data of glioblastoma multiforme U-87 cells exposed to targeted electromagnetic fields

A novel application of a non-invasive, electromagnetic field technology has a significant inhibitory effect on the proliferation of glioblastoma multiforme U-87 MG cells in culture. This study reports the cellular and molecular responses of U-87 MG cells to the effects of a tunable, non-ionizing radiation technology that does not induce the serious side effects commonly observed with chemotherapy. The broadband (RF/Low Microwave) electromagnetic field is tuned by means of oscillating wave forms, selected reference materials and a positive feedback loop (RGFIELDS™). By simultaneously targeting specific molecules (oligonucleotides and proteins) that contribute to pathogenesis of glioblastoma with this technology, the continuous exposure of cells for 54 h results in the inhibition of cell growth and a concurrent increase in cell death. We used microarrays to elucidate the cellular processes involved in the response of U-87 MG cells to exposure to RGFIELDS™ and identified mRNA and non-coding RNA sequences that are differentially modulated 1.5 fold or more. Total RNA was isolated from three independent biological replicates of U-87 MG cells exposed continuously for 54 h to RGFIELDS™ targeting selected reference materials (oligonucleotides and proteins), and from unexposed control cells cultured under identical conditions.

本研究展示了无创电磁场技术的全新应用，其可显著抑制培养状态下多形性胶质母细胞瘤（glioblastoma multiforme）U-87 MG细胞的增殖。本研究报道了U-87 MG细胞对一种可调谐非电离辐射技术的细胞与分子响应，该技术不会引发化疗常见的严重不良反应。该技术通过振荡波形、选定参考物质与正反馈环路（RGFIELDS™）对宽带（射频/低微波）电磁场进行调谐。借助本技术同时靶向参与多形性胶质母细胞瘤致病机制的特定分子（寡核苷酸与蛋白质），将细胞持续暴露于该环境54小时后，可实现细胞生长抑制与细胞死亡同步增加。本研究利用基因芯片（microarrays）解析U-87 MG细胞暴露于RGFIELDS™后的相关细胞过程，并鉴定出1.5倍及以上差异调控的信使RNA（mRNA）与非编码RNA（non-coding RNA）序列。本研究从两类样本中提取总RNA：一类为经三组独立生物学重复的、持续暴露于靶向选定参考物质（寡核苷酸与蛋白质）的RGFIELDS™环境54小时的U-87 MG细胞，另一类为相同培养条件下未暴露的对照细胞。