Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates

In this study, we set out to explore the genomic diversity of Kluyveromyces marxianus by analysing whole-genome data from 14 strains isolated from different sources. Some of these strains had been previously sequenced and published, here we deposit only those newly sequenced for this study. The results indicate a high degree of variation among isolates, in both ploidy and heterozygosity, and show a correlation between ploidy and environmental niche. Our work raises important questions about the life cycle of K. marxianus, and emphasizes the need to take ploidy and heterozygosity into account when considering using K. marxianus for biotechnological purposes.

本研究通过分析14株不同来源分离菌株的全基因组数据，旨在探究马克斯克鲁维酵母（Kluyveromyces marxianus）的基因组多样性。其中部分菌株此前已完成测序并发表，本研究仅提交本次新测序的菌株相关数据。研究结果表明，各分离株在倍性（ploidy）与杂合性（heterozygosity）两方面均存在显著变异，且倍性与生态位之间存在相关性。本研究提出了关于马克斯克鲁维酵母生命周期的重要科学问题，并强调：若将该菌应用于生物技术相关用途，需充分考虑其倍性与杂合性特征。