Comparative transcriptome analysis in response to heat stress in Arabidopsis thaliana and Arabidopsis lyrata

Arabidopsis thaliana and Arabidopsis lyrata are two closely related Brassicaceae species, which are used as models for plant comparative biology. They differ by lifestyle, predominant mating strategy, ecological niches and genome organization. To identify heat stress induced genes, we performed RNA-sequencing of rosette leaves from mock-treated, heat-stressed and heat-stressed-recoved plants of both species. Analysis of genetic element transcriptional changes in response to 6 hours of 37°C heat stress and 48 hours of recovery in Arabidopsis thaliana Col-0 and Arabidopsis lyrata MN47.

拟南芥（Arabidopsis thaliana）与 lyrata拟南芥（Arabidopsis lyrata）是两种亲缘关系紧密的十字花科（Brassicaceae）物种，二者均为植物比较生物学研究的经典模式物种。它们在生活方式、主要交配策略、生态位以及基因组组织结构上均存在显著差异。为鉴定热胁迫诱导基因，我们对两个物种的空白对照处理、热胁迫处理及热胁迫恢复处理植株的莲座叶开展了RNA测序（RNA-sequencing），并分析了拟南芥Col-0（Arabidopsis thaliana Col-0）与 lyrata拟南芥MN47（Arabidopsis lyrata MN47）在37℃热胁迫6小时、恢复培养48小时后各类遗传元件的转录变化。