Data_Sheet_3_Microbial community structure dynamics of invasive bullfrog with meningitis-like infectious disease.pdf

Meningitis-like infectious disease (MID) (also known as frog cataract and torticollis) is a disease prone to occur in amphibians and reptiles. It is highly contagious and has a high mortality rate. In this study, we sampled and sequenced microbiomes from oral and intestinal samples of five normal and five diseased bullfrogs. The analysis found that the richness, uniformity, and abundance of the microbial community of the diseased bullfrogs were significantly higher than those of the normal bullfrogs in both the oral cavity and the gut. In the diseased group, the abundance of Elizabethkingia significantly increased and that of Lactococcus significantly decreased. It showed that the structure of the microbial community had changed a lot in diseased frogs. After the pathogenic bacteria infected the body, it might be make the decline in the immune function of the body declined, and resulting in some conditional pathogenic bacteria in the water body further infecting the body. As a result, the richness and composition of the microbial community significantly changed. This study can provide a theoretical basis for the control of MID of bullfrogs.

类脑膜炎传染病（Meningitis-like infectious disease，简称MID，又称蛙白内障病、歪颈病）是一种多发于两栖类与爬行类动物的传染病，其传染性极强且致死率较高。本研究采集了5只健康牛蛙与5只患病牛蛙的口腔及肠道样本，并对其微生物组进行测序分析。结果显示，患病牛蛙口腔与肠道内的微生物群落丰富度、均匀度及丰度均显著高于健康牛蛙。在患病组中，伊丽莎白菌属（Elizabethkingia）的丰度显著升高，而乳球菌属（Lactococcus）的丰度则显著降低，表明患病牛蛙的微生物群落结构已发生显著改变。病原菌侵入机体后，可能会导致机体免疫功能下降，进而使水体中的条件致病菌进一步侵染机体，最终造成微生物群落的丰富度与组成发生显著变化。本研究可为牛蛙MID的防控提供理论依据。