Genome-wide maps of chromatin state in renal tubular epithelial cells

To further understand the underlying mechanisms and identify genes directly regulated by SETD2 and H3K36me3 on a genome-wide scale, we performed chromatin immunoprecipitation using a H3K36me3 specific antibody followed by next-generation sequencing (ChIP-seq) assays using the renal tubules isolated from SETD2FL/FL KSPCRE/+ and its control SETD2fl/fl mice. Overall design: Examination of 2 different histione modifaication in 2 primary renal tubular epithelial cells

为进一步在全基因组范围内阐明SETD2与H3K36me3的潜在调控机制，并筛选出受二者直接调控的靶基因，我们使用H3K36me3特异性抗体开展染色质免疫共沉淀（chromatin immunoprecipitation, ChIP）实验，随后对从SETD2^FL/FL KSPCRE/+小鼠及其对照SETD2^fl/fl小鼠中分离得到的肾小管进行下一代测序（next-generation sequencing, NGS），即染色质免疫共沉淀测序（ChIP-seq）。实验整体设计：对2种原代肾小管上皮细胞中的2种不同组蛋白修饰进行检测分析。