Chorioamnion after choriodecidual Group B Streptococcal infection. Macaca nemestrina

Choriodecidual infection is associated with preterm premature rupture of membranes (pPROM) and preterm birth. MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and may be involved in the pathway leading to chorioamnion weakening following infection. The study objective was to determine if a miRNA profile in the chorioamnion is associated with Group B Streptococcal infection and membrane weakening. Overall design: Ten chronically catheterized pregnant monkeys (Macaca nemestrina) at 118-125 days gestation (term=172 days) received one of two experimental treatments: 1) choriodecidual and intra-amniotic saline infusions (n=5), or 2) GBS choriodecidual inoculation (n=5).

绒毛膜蜕膜感染与早产胎膜早破（preterm premature rupture of membranes, pPROM）及早产存在关联。
微小RNA（miRNAs）是一类小型非编码RNA，可调控基因表达，可能参与感染后胎膜弱化的信号通路。
本研究旨在明确绒毛膜羊膜中的miRNA表达谱是否与B族链球菌（Group B Streptococcal）感染及胎膜弱化存在关联。
实验设计：选取10只妊娠118~125天（妊娠足月为172天）的经慢性导管植入的妊娠豚尾猴（Macaca nemestrina），随机分为两组，每组5只，分别接受以下两种实验处理之一：1）绒毛膜蜕膜及羊膜腔内生理盐水输注组（n=5）；2）B族链球菌（GBS）绒毛膜蜕膜接种组（n=5）。