Transcriptome profiling of spleen and duodenum upon lymphotoxin beta receptor (LTbR) induction

To study the effect of LTbR induction on spleen and duodenum transcriptome, we performed RNA sequencing of the spleen and intestine after Intravenous LTbR agonist AC H6 or isotype treatment. We analyzed differential gene expression. RNA sequencing on spleen and duodenum mRNA of wild type C57BL/6J mice treated with either AC H6 or isotype control for 24 hours (dose: 100ul of 1mg/ml solution per mouse, n=3 per genotype).

为探究淋巴毒素β受体（LTbR）诱导对脾脏与十二指肠转录组（transcriptome）的影响，我们对经静脉注射LTbR激动剂AC H6或同型对照（isotype control）处理后的脾脏与肠道样本开展了RNA测序（RNA sequencing），并进行差异基因表达分析。本研究针对经AC H6或同型对照处理24小时的野生型C57BL/6J小鼠的脾脏与十二指肠mRNA完成测序，给药方案为每只小鼠注射100μL浓度为1mg/mL的溶液，每组设置3个生物学重复。