MetaboLights MTBLS87 - GNPS Unexpected similarities between the Schizosaccharomyces and human blood metabolomes, and novel human metabolites (Blood fraction)

This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS87 Abstract:Metabolomics, a new branch of chemical biology, provides compositional and quantitative information about the state of organism or cell at the levels of metabolite constituents. We here report non-targeted metabolome of human blood that is made up of plasma and red blood cells (RBCs), using liquid-chromatography and mass spectrometry (LC-MS). Previously, metabolome of a microbe, the fission yeast Schizosaccharomyces pombe, was reported. Two sets of metabolome results are highly similar in their compositions: among 133 compounds identified in human blood, 101 (75%) are also present in S. pombe, and among 57 compounds enriched in RBC, 45 (78%) are also present in S. pombe. Most abundant metabolites are ATP, glutathione and glutamine. Many of other relatively abundant metabolites identified are also implicated in energy, anti-oxidant and amino acid metabolism. We show fourteen newly-identified blood compounds; citramalate, GDP-glucose, trimethyl-histidine, trimethyl-phenylalanine, trimethyl-tryptophan, trimethyl-tyrosine, UDP-acetyl-glucosamine, UDP-glucuronate, dimethyl-lysine, glutamate methyl ester, N-acetyl-(iso)leucine, N-acetyl-glutamate, N2-acetyl-lysine, and N6-acetyl-lysine (the first eight are enriched in RBC). Ten of them are also detected in S. pombe, and ten of them are methylated or acetylated, amino acids. Trimethylated or acetylated free amino acids are also abundant in white blood cell. Their physiologic role may be investigated in the future by yeast genetics.

本数据集下载自MetaboLights数据库（http://www.ebi.ac.uk/metabolights/），登录号为MTBLS87。
摘要：代谢组学作为化学生物学的新兴分支，可在代谢物组分层面提供生物体或细胞状态的组成与定量信息。本研究采用液相色谱-质谱联用技术（LC-MS），报道了由血浆与红细胞（RBCs）构成的人血液非靶向代谢组。此前已有研究报道了模式微生物粟酒裂殖酵母（Schizosaccharomyces pombe）的代谢组。两组代谢组结果的组成具有高度相似性：在人血液中鉴定出的133种化合物里，有101种（占比75%）同样存在于粟酒裂殖酵母中；而在红细胞中富集的57种化合物里，有45种（占比78%）同样存在于粟酒裂殖酵母中。丰度最高的代谢物为三磷酸腺苷（ATP）、谷胱甘肽与谷氨酰胺。本次鉴定出的其他多数丰度较高的代谢物，均参与能量代谢、抗氧化代谢与氨基酸代谢过程。本研究还鉴定出14种全新的人血液代谢物：柠苹酸、二磷酸鸟苷葡萄糖（GDP-glucose）、三甲基组氨酸、三甲基苯丙氨酸、三甲基色氨酸、三甲基酪氨酸、乙酰氨基葡糖二磷酸（UDP-acetyl-glucosamine）、葡糖醛酸二磷酸（UDP-glucuronate）、二甲基赖氨酸、谷氨酸甲酯、N-乙酰（异）亮氨酸、N-乙酰谷氨酸、N2-乙酰赖氨酸以及N6-乙酰赖氨酸（其中前8种物质在红细胞中富集）。其中10种代谢物同样在粟酒裂殖酵母中被检测到，且有10种为甲基化或乙酰化修饰的氨基酸。三甲基化或乙酰化修饰的游离氨基酸在白细胞中同样具有较高丰度。未来可通过酵母遗传学手段研究这些代谢物的生理功能。