Investigation of the methylation changes occurring during breast cancer pathology

The experiment was designed to discover deferentially methylated regions between DNA extracted from breast cancer tissues and DNA samples extracted from tissue obtained form breast reduction surgeries. The study involved 20 breast cancer samples and 5 tissue samples from breast reductions. Each of the samples was processed by the arrays and collected data were used to compare the genome wide methylation pattern cancer samples (cases) and breast reduction samples (controls). The methylation pattern of 5 DNA samples from breast reduction was compared with the methylation pattern of 20 DNA samples from breast cancer tumors

本实验旨在探究乳腺癌组织提取的DNA与乳房缩小手术获取的组织样本提取的DNA之间的差异甲基化区域（differentially methylated regions）。本研究共纳入20份乳腺癌组织样本与5份乳房缩小手术组织样本，所有样本均通过基因芯片完成检测，所得数据用于比较乳腺癌样本（病例组）与乳房缩小手术样本（对照组）的全基因组甲基化模式。本研究同时对比了5份乳房缩小手术来源的DNA样本与20份乳腺癌肿瘤来源的DNA样本的甲基化模式。