Gene expression analysis of murine SAMHD1 deficient peritoneal macrophages

To elucidate responses of myeloid cells to SAMHD1 deficiency in the absence of exogenous viral infection, we performed global gene expression analysis of SAMHD1 deficient macrophages. Overall design: Peritoneal macrophages from nine mutants and nine controls were FACS sorted. Cells from three animals were pooled to yield three poolls per group. RNA from these pools was subjected to next generation mRNA sequencing

为阐明无外源病毒感染条件下髓系细胞对SAMHD1缺陷的应答特征，我们对SAMHD1缺陷型巨噬细胞开展了全基因组基因表达分析。
实验整体设计：从9只突变体小鼠与9只对照小鼠中分离腹腔巨噬细胞，经荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）富集纯化。将每3只动物的细胞合并为一个样本，每组共获得3个混合样本。随后对上述混合样本的RNA进行下一代mRNA测序（next generation mRNA sequencing）。