Gene expression profile of replicative senescence in normal human diploid fibroblasts.. Homo sapiens

Transcriptional profiling of normal human diploid fibroblasts(TIG-3) comparing late passage with early passage. Overall design: Total RNA was isolated using TRIzol reagent and were analyzed using the human 3D-Gene DNA chip (Toray) that contains 25000 genes. The genome wide transcriptional response of senescent cells(more than 70 population doublings)(Replicative) were compared to that of proliferating cells(less than 40 population doublings)(Young).

正常人类二倍体成纤维细胞（TIG-3）的转录谱分析（Transcriptional profiling）：对比晚期传代与早期传代细胞。实验设计：使用TRIzol试剂提取总RNA，采用涵盖25000个基因的人类3D-Gene DNA芯片（Toray）进行分析。将复制性衰老细胞（群体倍增数超过70代）的全基因组转录响应，与增殖期细胞（群体倍增数低于40代，即年轻细胞）进行对比。