Genome-wide EML1 binding sites in Arabidopsis thaliana seedlings

To identify genomic targets involved in the EML-dependent control of seed development, we performed ChIP-chip using whole genome Arabidopsis tiling arrays from p35S::EML1-GFP seedlings using GFP antibodies. The analysis showed that EML1 displayed preferential binding to transposable elements (TEs), while showing no preference for protein coding genes. 1 input and 1 ChIP sample

为鉴定参与EML依赖型种子发育调控的基因组靶标，我们以p35S::EML1-GFP转基因拟南芥幼苗为材料，采用绿色荧光蛋白（GFP）抗体，使用拟南芥全基因组平铺芯片（whole genome Arabidopsis tiling arrays）开展染色质免疫沉淀芯片（ChIP-chip）实验。分析结果表明，EML1对转座因子（transposable elements, TEs）具有优先结合特性，而对蛋白质编码基因无显著结合偏好。本次实验共设置1份输入对照样本与1份ChIP样本。