Genome–wide gene expression profile of adipocytes and infiltration macrophages obtained from abdominal (visceral and subcutaneous) and peripheral (thigh) adipose depots from Normal Glucose Tolerant and Type 2 Diabetics Asian Indians

We have identified molecular-level alternations in different adipose depots (thigh, visceral and subcutaneous) of Asian Indians (both male and female) suffering from type-2 diabetes as compared to age and BMI matched normal glucose tolerant subjects by functional analysis of differentially expressed genes, and correlation of gene expression estimates with measured intermediate traits associated with T2D and its related co-morbidities (Hb1Ac, HOMA-B, HOMA-R, NEFA, Triglyceride, Total Cholesterol, HDL, LDL, VLDL, Leptin, Adiponectin, TNF-α, Serum- Creatinine, IL-6, High sensitivity - serum-creatinine (hs-CRP) and also size of adipocytes). Funding: Grant Id: 5/4/8/2012-RMC Grant title: Clinical, Biochemical and Cellular Correlates of Transcriptome of Adipose Tissue Among Type-2 Diabetics Name of the funding source: Indian Council for Medical Research We have recruited 30 controls & 30 diabetic subjects undergoing femur bone surgery and 16 controls & 19 diabetic subjects undergoing abdominal surgery (two samples from each subject, one subcutaneous and one visceral fat biopsy were extracted). So total 130 samples were obtained and analyzed for genome –wide gene expression profile of Adipocytes and infiltration macrophages from three different depots of adipose tissue.

本研究通过对差异表达基因（differentially expressed genes）开展功能分析，并将基因表达估算值与2型糖尿病（T2D）及其相关合并症相关的检测中间表型——包括糖化血红蛋白（Hb1Ac）、稳态模型评估β细胞功能（HOMA-B）、稳态模型评估胰岛素抵抗（HOMA-R）、非酯化脂肪酸（NEFA）、甘油三酯（Triglyceride）、总胆固醇（Total Cholesterol）、高密度脂蛋白胆固醇（HDL）、低密度脂蛋白胆固醇（LDL）、极低密度脂蛋白胆固醇（VLDL）、瘦素（Leptin）、脂联素（Adiponectin）、肿瘤坏死因子α（TNF-α）、血清肌酐（Serum-Creatinine）、白细胞介素6（IL-6）、高敏C反应蛋白（原文标注为High sensitivity - serum-creatinine，对应hs-CRP）以及脂肪细胞大小——进行关联分析，明确了亚裔印度男性与女性2型糖尿病患者大腿部、内脏及皮下三种脂肪沉积部位（adipose depots）的分子水平改变，并与年龄和BMI匹配的糖耐量正常受试者进行对比。
资助信息：项目编号为5/4/8/2012-RMC，项目名称为《2型糖尿病患者脂肪组织转录组的临床、生化与细胞关联研究》，资助方为印度医学研究理事会（Indian Council for Medical Research）。
本研究共招募接受股骨手术的对照受试者30名、2型糖尿病患者30名，以及接受腹部手术的对照受试者16名、2型糖尿病患者19名；每名受试者采集两份活检样本，分别为皮下脂肪组织与内脏脂肪组织。最终共获得130份样本，针对来自三种不同脂肪沉积部位的脂肪细胞及浸润巨噬细胞的全基因组基因表达谱（genome-wide gene expression profile）进行分析。