Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco

Three tobacco samples that have been enriched for the mitochondria (MT) were sequenced on a PacBio Sequel IIe in HiFi-CCS mode. Sequences in the vicinity of the essential gene atp1 in the mitochondrial genomes have been edited. The HiFi reads for the three samples were mapped to reference tobacco MT genome BA000042 using Minimap2 (version 2.24-r1122) with the flags -ax map-hifi --sam-hit-only. The read identifiers for the reads that mapped to BA000042 were then saved and used to select a subset of the raw reads. The read subset for each sample was assembled using hifiasm (version 0.16.1-r375) with the -l 0 flag and the assembled contigs can be found in the fasta files. In select cases, trimmed, hand-curated contigs are also shown in addition to the original assembly fasta file.

本数据集涉及3株经线粒体（mitochondria, MT）富集处理的烟草样本，采用PacBio Sequel IIe平台的HiFi-CCS模式完成测序。线粒体基因组中核心必需基因atp1的侧翼序列已完成编辑。利用Minimap2（版本2.24-r1122）及参数`-ax map-hifi --sam-hit-only`，将三个样本的HiFi reads比对至烟草线粒体参考基因组BA000042。随后提取比对至BA000042的reads的序列标识，以此从原始reads中筛选得到目标reads子集。针对每个样本的reads子集，采用hifiasm（版本0.16.1-r375）并指定参数`-l 0`完成组装，组装得到的重叠群（contigs）可在FASTA格式文件中获取。在部分特定场景下，除原始组装得到的FASTA格式文件外，还提供经过序列修剪与人工精修的重叠群结果。