Hyphthalamic mRNA expression of leptin receptor-expressing cell-specific Slug knockout mice. Hyphthalamic mRNA expression of leptin receptor-expressing cell-specific Slug knockout mice

Leptin resistance is a driving force for obesity and metabolic syndrome. Slug (Snai2 or Snail2) is an epignetic factor that may play a critical role in the development of leptin resistance in obesity. We conducted a microarray to detect molecular targets of Slug in the hypothalamus. Overall design: Hypothalamic RNAs were extracted from leptin receptor-expressing cell-specific Slug knockout (ΔLepR) or Slugfl/fl male mice at 8 weeks of age and subjected to Affymetrix microarray analysis.

瘦素抵抗（leptin resistance）是肥胖与代谢综合征的驱动因素。Slug（Snai2或Snail2）作为一种表观遗传因子，可能在肥胖相关瘦素抵抗的发生发展中发挥关键作用。本研究通过基因芯片（microarray）技术检测下丘脑中Slug的分子靶标。实验整体设计：选取8周龄的表达瘦素受体的细胞特异性Slug敲除（ΔLepR）雄性小鼠与Slugfl/fl雄性小鼠，提取其下丘脑RNA，随后开展Affymetrix基因芯片分析。