Transcriptome analysis of lung epithelial cells and lung fibroblasts from various developmental stages (E18.5, P0.5, P2, P7, P28, and P56)

In the alveoli, lung fibroblasts are in close contact with alveolar epithelial cells type 2, and are considered to support alveolar epithelial cells, forming an alveolar stem cell niche. However, what fibroblast-to-epithelial cell interactions occur during the alveolar maturation stage remains unclear. To understand the lung fibroblast-to-epithelial cell interactions, we performed time-course 3´SAGE-seq analysis of lung epithelial cells and fibroblasts. Lung epithelial cells and lung fibroblasts from various developmental stages (E18.5, P0.5, P2, P7, P28, and P56) were purified by cell sorting. The time series transcriptome of the epithelial cells and fibroblasts was generated by 3'SAGE-seq using Ion Proton sequencer.

在肺泡微环境中，肺成纤维细胞（lung fibroblasts）与肺泡II型上皮细胞（alveolar epithelial cells type 2）紧密接触，被认为可对后者发挥支持作用，并共同构成肺泡干细胞龛（alveolar stem cell niche）。然而，在肺泡成熟阶段，成纤维细胞与上皮细胞之间的相互作用机制仍未明确。为阐明肺成纤维细胞与上皮细胞间的相互作用机制，我们对肺上皮细胞及成纤维细胞开展了时序性3´SAGE-seq分析。我们通过细胞分选技术，纯化获得不同发育阶段（E18.5、P0.5、P2、P7、P28及P56）的肺上皮细胞与肺成纤维细胞。本研究采用Ion Proton测序仪，通过3´SAGE-seq技术构建了上皮细胞与成纤维细胞的时序转录组数据集。