Bulk transcriptome analysis of Myt1l mutant mouse neuron primary cultures.

We examined the effect of Myt1l deficiency in the neurons of mice. Homozygous Myt1l deficiency resulted in postnatal lethality, and mutant mice presented gene expression changes associated with developmental delays and resembled changes observed in autism spectrum disorder patients. Bulk RNA sequencing of Myt1l (+/+; WT) and Myt1l (+/-; HET) mouse cortical neuron primary cultures from P0 mice.

本研究探究了Myt1l基因缺陷对小鼠神经元的影响。纯合型Myt1l缺陷可导致小鼠出生后致死，突变小鼠出现与发育迟缓相关的基因表达改变，且其表达谱变化与自闭症谱系障碍（Autism Spectrum Disorder, ASD）患者体内的改变高度相似。本研究对取自出生后0天（Postnatal Day 0, P0）小鼠的Myt1l野生型（+/+; WT）与杂合型（+/-; HET）皮质神经元原代培养物开展了批量RNA测序（Bulk RNA sequencing）。