4C-seq analysis of NIPBL depletion in melanoma cell line

Genomic interactions of ALK ATI or ULK4 intron 31 viewpoint was studied after knockdown of NIPBL in melanoma cell lines 501mel 4C-seq was performed from ALK ATI or ULK4 intron 31 viewpoint on shLuc or shNIPBL_#2 knockdown cells for 501mel

本研究以黑色素瘤细胞系501mel为模型，探究了NIPBL基因敲低后，以ALK ATI或ULK4内含子31为锚定位点的基因组相互作用；实验中，我们对该细胞系的靶向荧光素酶短发夹RNA（shLuc）转染组与靶向NIPBL的2号短发夹RNA（shNIPBL_#2）转染敲低组，以ALK ATI或ULK4内含子31为锚定位点开展了4C-seq测序实验。