Absolute expression level of Saccharomyces paradoxus genes. Saccharomyces paradoxus

Total RNA versus genomic DNA hybridization on custom arrays designed for all Saccharomyces paradoxus genes Overall design: Total RNA was collected in mid-log phase from Saccharomyces paradoxus cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Saccharomyces paradoxus.

针对所有奇异酵母（Saccharomyces paradoxus）基因设计的定制微阵列上的总RNA与基因组DNA杂交实验
实验设计概况：从采用实验室自制配方的丰富培养基（缩写为CM）中培养的奇异酵母细胞的对数中期阶段收集总RNA。随后将总RNA反转录为互补DNA（cDNA），经Cy3荧光标记后，与经Cy5荧光标记的奇异酵母基因组DNA进行竞争性杂交。