autologous pairs of cutaneous melanocyte and melanoma cell cultures. Homo sapiens

Early-passage (<10 passages) cultures of melanoma cells from metastatic lymph node lesions and normal adult melanocytes explanted in parallel from the adjacent, non-involved skin of 5 patients were compared by cDNA arrays. Differences between normal and neoplastic counterparts were then assessed upon adjustment for individual factors. Overall design: cDNA expression profiles comparison between normal and neoplastic counterparts in a strictly autologous setting

本研究采用cDNA芯片（cDNA arrays），对5例患者转移性淋巴结病灶来源的黑色素瘤细胞早期传代（传代次数<10代）培养物，以及同期从其邻近未受累皮肤分离培养的正常成人黑素细胞进行了对比分析。随后在校正个体因素后，评估了正常细胞与肿瘤对应细胞之间的表达差异。实验整体设计：在严格自体配对体系下，对比正常细胞与肿瘤对应细胞的cDNA表达谱。