Interaction of Catechu Dye with DNA: Spectroscopic and In Silico Approach

Catechin, a yellow colored molecule obtained from the wood of Acacia catechu was analyzed for its interaction with synthetic DNA duplexes using spectroscopic analysis. UV-Visible spectroscopic analysis revealed the non-intercalative binding mode. Fourier Transform Infrared spectroscopy (FTIR) analysis expose chemical shift indicated by various vibrational stretches and an increase in the intensity of base stacking was observed by Circular Dichroism (CD), respectively. This inference was further confirmed through nuclear staining technique and also in electrophoretic technique; the dye quenches the fluorescent intensity of ethidium bromide. The result of fluorescence spectroscopy was in concordance with the electrophoretic technique. In addition, the spectroscopic results were in accordance with the molecular docking studies of specific catechin compound from the catechu dye with CT-DNA. This kind of site specificity is a gain in the medicinal field as the drug can be DNA targeted for cancer therapeutics. The present work reveals that catechu dye has a noteworthy application in the field of medical bioscience.

从儿茶树（Acacia catechu）木材中提取的黄色小分子化合物儿茶素（Catechin），本研究借助光谱分析法探究了其与合成DNA双链体的相互作用。紫外-可见光谱分析显示，二者结合模式为非插入式结合。傅里叶变换红外光谱（FTIR）分析揭示了多种振动伸缩峰对应的化学位移变化，圆二色谱（CD）检测则观察到碱基堆积强度显著升高。上述结论进一步通过核染色技术与电泳技术得到验证：该化合物可淬灭溴化乙锭（Ethidium Bromide）的荧光强度。荧光光谱实验结果与电泳技术检测结果一致。此外，光谱分析结果与儿茶提取物中特定儿茶素类化合物与CT-DNA的分子对接研究结果相符。这种位点特异性结合特性在医药领域具备重要应用价值，可为靶向DNA的癌症治疗药物开发提供思路。本研究表明，儿茶提取物在医学生命科学领域拥有值得关注的应用潜力。