Rhodococcus aerolatus strain:js360 Genome sequencing. Rhodococcus aerolatus strain:js360

4-Nitroaniline (4NA), the starting material for the first synthesized azo dye, is a toxic compound found in industrial wastewaters . Several bacterial strains capable of 4NA biodegradation were previously reported but the details of the catabolic pathway were not established. We isolated a Rhodococcus sp. by selective enrichment from 4NA-contaminated soil. When grown on 4NA, the isolate released a stoichiometric amount of nitrite followed by less than stoichiometric ammonia release. Enzyme assays coupled with respirometry provided preliminary evidence that the first and second steps of 4NA degradation involve monooxygenase catalyzed reactions followed by ring cleavage prior to deamination. Sequencing and annotation of the whole genome revealed candidate monooxygenases

4-硝基苯胺（4-Nitroaniline, 4NA）作为首款人工合成偶氮染料的起始原料，是一种存在于工业废水中的有毒化合物。此前已有多篇文献报道了具备4NA生物降解能力的细菌菌株，但该降解过程的分解代谢通路细节尚未阐明。本研究通过选择性富集培养法，从受4NA污染的土壤中分离得到一株红球菌属（Rhodococcus）菌株。当以4NA为生长底物培养该分离菌株时，其会释放出化学计量当量的亚硝酸盐，随后释放的氨量则低于化学计量值。酶活测定结合呼吸计量法的初步实验结果表明，4NA降解的前两步依次为单加氧酶（monooxygenase）催化的反应，随后在脱氨作用前发生芳环裂解。对全基因组进行测序与注释分析后，发现了候选的单加氧酶编码基因。