Exposure to low-oxygen environment cause implantation failure and changes in transcriptome of uterus and ovary. Exposure to low-oxygen environment cause implantation failure and changes in transcriptome of uterus and ovary

Hypoxia is a condition in which tissues of the body do not receive sufficient amounts of oxygen supply. The current study aimed to clarify the impact of a forced low-oxygen environment on the early pregnancy by exposing mice to low-oxygen conditions for 72 hours after fertilization. The treatment resulted in a complete failure of blastocyst implantation accompanied by vascular hypermeability in the uterus. The transcriptome analysis of uterus and ovary revealed remarkable alterations in gene expression between the control normoxic and hypoxic treatment groups. These alterations were encompassed a number of genes categorized to the immune responses and oxidative demethylation process. Overall design: To explore the effect of forced low oxygen on the implantation window. Pregnant mice exposed to low oxogen 72 hours after fertilization the then were sacrificied at E3.5, Uterine and ovarian samples were collected. Total RNA were wxtracted for each treatment group (hypoxia and normoxia), three samples were pooled, and RNA sequencing libraries were constructed. Differentially expressed genes (DEGs) were identified based on a Log2-fold change (FC) threshold of ≥ 2.

缺氧（Hypoxia）是指机体组织无法获得充足氧气供应的病理状态。本研究旨在阐明强制性低氧环境对早期妊娠的影响，具体方法为使小鼠在受精后暴露于低氧环境72小时。实验结果显示，该处理导致囊胚着床完全失败，并伴随子宫血管通透性升高。对子宫与卵巢开展的转录组分析（transcriptome analysis）显示，常氧对照组与低氧处理组间的基因表达存在显著改变，这些改变涉及参与免疫应答与氧化去甲基化过程的多个基因。总体实验设计：为探究强制性低氧对胚胎着床窗口期的影响，将受精72小时后的孕鼠暴露于低氧环境，并于胚胎发育第3.5天（E3.5）处死小鼠，收集子宫与卵巢样本。提取各组（低氧组与常氧对照组）总RNA，将三份样本混合后构建RNA测序文库。基于Log2倍数变化（FC）阈值≥2筛选差异表达基因（Differentially expressed genes, DEGs）。