Compartment-specific investigation of differential gene expression profiles in lungs with idiopathic pulmonariy fibrosis (IPF)

Lung tissues were collected from patients with IPF undergoing lung transplantation. Non-transplanted donor lung tissue showing no evidence of interstitial lung disease served as healthy controls. Septa from different regions of the lungs were mircodissected and their RNA was subjected to microarray-analysis. RNA from 10 heathy donor tissues was pooled and used as a pooled reference. From each of the 10 IPF patients, samples were collected once from “healthy looking” (non-fibrotic) regions and from fibrotic loci. Two-color design, 3 groups: one pooled healthy control sample (from n=10 patients), 10 samples from septa from normal and from fibrotic of n=10 patients.

本数据集的样本取自接受肺移植的特发性肺纤维化（Idiopathic Pulmonary Fibrosis, IPF）患者的肺组织。以无肺间质病（Interstitial Lung Disease, ILD）证据的未移植供体肺组织作为健康对照。对肺部不同区域的肺间隔组织进行显微解剖，提取其RNA并开展基因芯片分析（microarray analysis）。将10份健康供体肺组织的RNA混合，作为混合对照参考样本。从10名特发性肺纤维化患者中，各采集1份"外观健康"（非纤维化）区域及纤维化病灶处的肺间隔组织样本。本实验采用双色杂交设计，共设置3组样本：1份混合健康对照样本（源自10名供体），以及10名患者的正常肺间隔与纤维化肺间隔组织样本。