Honey bees respond to multimodal stimuli following the Principle of Inverse Effectiveness_Dataset

Readme file for JEB243832 Journal of Experimental Biology (2022) 225, jeb243832. doi:10.1242/jeb.243832 Honey bees respond to multimodal stimuli following the Principle of Inverse Effectiveness Oswaldo Gil-Guevara1, *, Hernan A. Bernal2 and Andre J. Riveros1,3, * 1Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario. Cra. 26 #63B-48. Bogotá. Colombia. Bogotá, Colombia 2Programa de Ingeniería Biomédica, Escuela de Medicina y Ciencias de la Salud, Universidad del Rosario. Bogotá, Colombia 3Department of Neuroscience. University of Arizona, Tuczon, AZ, 85721 USA. * Corresponding authors: 1*O.G., 1*O.G., [e-mail] 1,3*A.J.R., [e-mail] ; [e-mail] Readme: Data was collected using a variation of the conditioning of the proboscis extension response (PER) protocol (Giurfa and Sandoz, 2012; Matsumoto et al., 2012). We adapted a training apparatus that allows both precise and automatic delivery of olfactory and visual stimuli (Fig. 1A) (Jernigan et al., 2014; Riveros and Gronenberg, 2009; Riveros et al., 2020). We employed custom software in Processing and Arduino UNO to control airflow output from a set of pumps limited by electronic valves and electric currents connected to a set of LED lights (see methods). Data was processed directly into excel sheets and from there into R (RStudio) (R Core Team, 2021). The first three columns are categorical variables describing treatment distribution. The first column represents the treatments employed divided into modalities (olfaction, visual and bimodal) at three different intensities (low, mid, and high). The second column divides data into modalities (without differentiating the intensity) and the third column describes intensity (without differentiating the modality). The fourth column counts the number of individuals per individual treatment; the fifth column counts the consecutive number of individuals employed in the experiments. The sixth column represents the consecutive number of trials in which individual bees received the stimuli during the PER protocol. The seventh column shows the binary response (PER response). Finally, the eight-column shows the latency time to PER response.

# 《实验生物学杂志》(Journal of Experimental Biology) 2022年第225卷JEB243832号论文自述文件
DOI: 10.1242/jeb.243832
论文标题：《蜜蜂遵循逆有效性原则（Principle of Inverse Effectiveness）对多模态刺激（multimodal stimuli）作出响应》
作者：Oswaldo Gil-Guevara¹, *, Hernan A. Bernal², Andre J. Riveros¹,³, *
¹ 罗萨里奥大学生物系，自然科学学院，哥伦比亚波哥大，Carrera 26 #63B-48
² 罗萨里奥大学医学院与健康科学学院生物医学工程专业，哥伦比亚波哥大
³ 亚利桑那大学神经科学系，美国亚利桑那州图森市，85721
* 通讯作者：¹*O.G.（电子邮箱：[e-mail]），¹,³*A.J.R.（电子邮箱：[e-mail]；[e-mail]）

数据集说明
本研究的数据采集基于喙伸反射（Proboscis Extension Response, PER）条件化范式的改良版本（Giurfa与Sandoz, 2012; Matsumoto等, 2012）。我们改装了一套训练装置，可精准且自动化地递送嗅觉与视觉刺激（图1A）（Jernigan等, 2014; Riveros与Gronenberg, 2009; Riveros等, 2020）。实验采用基于Processing与Arduino UNO开发的定制软件，通过电子阀门与电流控制泵组的气流输出，并联动一组LED灯（详细方法参见论文方法部分）。

数据先整理至Excel表格，随后导入R（RStudio）进行统计分析（R Core Team, 2021）。数据集各列的含义如下：
1. 第1列为分类变量，描述处理组的分布情况，涵盖3种刺激模态（嗅觉、视觉、双模态），且每种模态分为低、中、高3种强度；
2. 第2列按刺激模态分组（不区分强度）；
3. 第3列按刺激强度分组（不区分模态）；
4. 第4列为各处理组的受试个体数量；
5. 第5列为实验中使用的连续受试个体总数；
6. 第6列为单只蜜蜂在PER范式中接受刺激的连续试验次数；
7. 第7列为二元响应变量（即是否出现PER响应）；
8. 第8列为PER响应的潜伏期时长。